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Murine pulmonary microvascular endothelial cells (MPMEC) isolated from homozygous mutant mice express no endogenous EPHA2 protein. MPMEC show impaired ephrin-A1-induced vascular assembly and defective migration both in vitro and in vivo.

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In addition, MPMEC from homozygous mice exhibit decreased angiogenesis and fail to activate Rac1 in response to ephrin-A1 in vivo. Mutant mice crossed to BALB/c for 7 generations have been shown to exhibit protection from tumor progression, angiogenesis and metastasis following metastatic mammary adenocarcinoma cell transplantation. Mice homozygous for this targeted mutation are viable and fertile with no overt developmental or behavioral abnormalities. The Jackson Laboratory is distributing these mice on their original C57BL/6;129S6 mixed background. They may be useful in studies related to postnatal angiogenesis (endothelial cell migration, assembly into new tubules, and cytoskeletal regulation), as well as adenocarcinoma and breast cancer research.

A targeting vector was designed to insert a bacterial neo casette into exon 5 (nucleotide 1372, corresponding to amino acid residue 426 in the extracellular domain) of the endogenous gene. This construct was microinjected into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females for one generation.

Heterozygous offspring were interbred to produce homozygous mice. Mutant mice were maintained by breeding non-agouti siblings for more than 10 years before arrival at The Jackson Laboratory. Allele Symbol: Allele Name targeted mutation 1, Joseph C Ruiz Allele Type Targeted (Null/Knockout) Allele Synonym(s) targeted mutation 1, Joseph C Ruiz; Epha2 tm1Jrui Gene Symbol and Name, Eph receptor A2 Gene Synonym(s) CTPA; CTPP1; CTRCT6; epithelial cell kinase; ECK; Eck; ARCC2; expressed sequence AW545284; Sek-2; Sek2; Myk2; AW545284; Eck Strain of Origin 129S/SvEv-Gpi1 c Chromosome 4 Molecular Note A neo cassette was inserted at nucleotide 1372, within the sequence encoding the extracellular domain. Although the resultant transcript putatively encodes a nonfunctional protein truncated at amino acid 426, no protein was detected by Western blot analysis of murine pulmonary microvascular endothelial cells from mutant mice.

Mutations Made By Joseph Ruiz, Indiana University School of Medicine. Cryorecovery - Pricing Heterozygous for Epha2 We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered.

While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks.

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If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility.

If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. Payment Terms and Conditions Terms are granted by individual review and stated on the customer invoice(s) and account statement.

These transactions are payable in U.S. Currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings. The Jackson Laboratory's Genotype Promise The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards.

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